96 Wells Human IL-6 ELISA Kit

MultiSciences

70-EK106

Product Name

Human IL-6 ELISA Kit

Customs Name

Human IL-6 ELISA Kit

Application

ELISA

Reactivity

Human

Assay Type

Sandwich ELISA

Suitable Sample Type

serum, plasma, cell culture supernates

Format

96-well strip plate

Storage

4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)

Shipping Condition

4℃

Sample Volume

50 μl

Sensitivity

0.37 pg/ml

Standard Curve Range

7.81 - 500 pg/ml

Spike Recovery Range

99 % - 115 %

Mean Spike Recovery

1.06

CV of Intra plate

2.5 % - 5.0 %

CV of Inter plate

1.8 % - 4.4 %

Release Date

Components

96-well polystyrene microplate coated with a monoclonal antibody against IL-6

Human IL-6 Standard, lyophilized

IL-6 Detect Antibody

Standard Diluent

Streptavidin-HRP

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

washing Buffer (20×)

Plate Covers

"This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-6. The Human IL-6 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. In humans, it is encoded by the IL-6 gene. IL-6 is secreted by T cells and macrophages to stimulate immune response, e.g. during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6 also plays a role in fighting infection, as IL-6 has been shown in mice to be required for resistance against bacteriumStreptococcus pneumoniae.

IL-6 stimulates the inflammatory and auto-immune processes in many diseases such as diabetes, atherosclerosis, depression, Alzheimer's Disease, systemic lupus erythematosus, multiple myeloma, prostate cancer, Behçet's disease, and rheumatoid arthritis. Advanced/ metastatic cancer patients have higher levels of IL-6 in their blood."